Isolation and production of polyhydroxybutyrate (PHB) from Bacillus pumilus NMG5 strain for bioplastic production and treatment of wastewater from paper factories

Polyhydroxybutyrate (PHB) has the potential to replace traditional plastics and limit environmental pollution caused by plastic waste. This study combined wastewater treatment with PHB production reduce costs. Bacteria capable of synthesizing were isolated from paper mill identified using Matrix Assisted Laser Desorption/Ionization–Time Flight (MALDI-TOF) mass spectrometry 16S rRNA gene analysis. Bacillus pumilus NMG5 strain was found have a good yield in modified Nutrient Broth culture, reaching 42.28% dry biomass. The product analyzed FTIR spectroscopy 1H NMR spectroscopy. bacterial also tested for its ability treat wastewater, it showed impressive results terms biochemical oxygen demand (COD), total nitrogen, phosphorus, efficiencies 95.93%, 79.36%, 83.55%, respectively. that promising solution costs pollution. B. had high PHB, quality, as confirmed Furthermore, treating COD, phosphorus efficiencies. These suggest this harmless bacterium could be used systems produce providing sustainable environmentally friendly solution.